› 7tff

X-ray diffraction
3.6Å resolution

Crystal structure of human platelet phosphofructokinase-1 mutant- D564N

Released:
DOI: 10.2210/pdb7tff/pdb
PDB entry 7tff coloured by chain, front view.
PDB entry 7tff coloured by chain, side view.
PDB entry 7tff coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-associated somatic mutations in human phosphofructokinase-1 reveal a critical electrostatic interaction for allosteric regulation of enzyme activity.
Voronkova MA, Hansen HL, Cooper MP, Miller J, Sukumar N, Geldenhuys WJ, Robart AR, Webb BA
OpenAccess logo Biochem J 480 1411-1427 (2023)
PMID: 37622331

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, platelet type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 812 amino acids
Theoretical weight: 89.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q01813 (Residues: 1-784; Coverage: 100%)
Gene names: PFKF, PFKP
Sequence domains: Phosphofructokinase

Ligands and Environments

2 bound ligands:
Ligand PO4 8 x PO4
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 77.889Å b: 159.655Å c: 131.248Å
α: 90° β: 104.008° γ: 90°
R-values:
R R work R free
0.254 0.25 0.307
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Hitting the Sweet Spot: How Glucose Metabolism Is Orchestrated in Space and Time by Phosphofructokinase-1.
Campos et al. (2023)