› 7tos

Electron Microscopy
2.9Å resolution

E. coli 70S ribosomes bound with the ALS/FTD-associated dipeptide repeat protein PR20

Released:
DOI: 10.2210/pdb7tos/pdb
PDB entry 7tos coloured by chain, front view.
PDB entry 7tos coloured by chain, side view.
PDB entry 7tos coloured by chain, top view.
Source organisms:
Primary publication:
Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
Loveland AB, Svidritskiy E, Susorov D, Lee S, Park A, Zvornicanin S, Demo G, Gao FB, Korostelev AA
OpenAccess logo Nat Commun 13 2776 (2022)
PMID: 35589706
Related structures: EMD-26037

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
PDBe Complex ID:
PDB-CPX-199572 (preferred)
Entry contents:
53 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (56 distinct):
Large ribosomal subunit protein uL2 Chain: L02
Molecule details ›
Chain: L02
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL3 Chain: L03
Molecule details ›
Chain: L03
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL4 Chain: L04
Molecule details ›
Chain: L04
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL5 Chain: L05
Molecule details ›
Chain: L05
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL6 Chain: L06
Molecule details ›
Chain: L06
Length: 176 amino acids
Theoretical weight: 18.8 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL9 Chain: L09
Molecule details ›
Chain: L09
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL10 Chain: L10
Molecule details ›
Chain: L10
Length: 131 amino acids
Theoretical weight: 14.11 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL11 Chain: L11
Molecule details ›
Chain: L11
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL13 Chain: L13
Molecule details ›
Chain: L13
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL14 Chain: L14
Molecule details ›
Chain: L14
Length: 122 amino acids
Theoretical weight: 13.45 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL15 Chain: L15
Molecule details ›
Chain: L15
Length: 143 amino acids
Theoretical weight: 14.88 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL16 Chain: L16
Molecule details ›
Chain: L16
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL17 Chain: L17
Molecule details ›
Chain: L17
Length: 120 amino acids
Theoretical weight: 13.72 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL18 Chain: L18
Molecule details ›
Chain: L18
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL19 Chain: L19
Molecule details ›
Chain: L19
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL20 Chain: L20
Molecule details ›
Chain: L20
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL21 Chain: L21
Molecule details ›
Chain: L21
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL22 Chain: L22
Molecule details ›
Chain: L22
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL23 Chain: L23
Molecule details ›
Chain: L23
Length: 93 amino acids
Theoretical weight: 10.55 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL24 Chain: L24
Molecule details ›
Chain: L24
Length: 102 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL25 Chain: L25
Molecule details ›
Chain: L25
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL27 Chain: L27
Molecule details ›
Chain: L27
Length: 75 amino acids
Theoretical weight: 8.17 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL28 Chain: L28
Molecule details ›
Chain: L28
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL29 Chain: L29
Molecule details ›
Chain: L29
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL30 Chain: L30
Molecule details ›
Chain: L30
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL31 Chain: L31
Molecule details ›
Chain: L31
Length: 66 amino acids
Theoretical weight: 7.52 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL32 Chain: L32
Molecule details ›
Chain: L32
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL33 Chain: L33
Molecule details ›
Chain: L33
Length: 50 amino acids
Theoretical weight: 5.81 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL34 Chain: L34
Molecule details ›
Chain: L34
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL35 Chain: L35
Molecule details ›
Chain: L35
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL36A Chain: L36
Molecule details ›
Chain: L36
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS2 Chain: S02
Molecule details ›
Chain: S02
Length: 225 amino acids
Theoretical weight: 25.02 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS3 Chain: S03
Molecule details ›
Chain: S03
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS4 Chain: S04
Molecule details ›
Chain: S04
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS5 Chain: S05
Molecule details ›
Chain: S05
Length: 157 amino acids
Theoretical weight: 16.53 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS6, non-modified isoform Chain: S06
Molecule details ›
Chain: S06
Length: 100 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS7 Chain: S07
Molecule details ›
Chain: S07
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS8 Chain: S08
Molecule details ›
Chain: S08
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS9 Chain: S09
Molecule details ›
Chain: S09
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS10 Chain: S10
Molecule details ›
Chain: S10
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS11 Chain: S11
Molecule details ›
Chain: S11
Length: 116 amino acids
Theoretical weight: 12.39 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS12 Chain: S12
Molecule details ›
Chain: S12
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS13 Chain: S13
Molecule details ›
Chain: S13
Length: 114 amino acids
Theoretical weight: 12.63 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS14 Chain: S14
Molecule details ›
Chain: S14
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS15 Chain: S15
Molecule details ›
Chain: S15
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS16 Chain: S16
Molecule details ›
Chain: S16
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS17 Chain: S17
Molecule details ›
Chain: S17
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS18 Chain: S18
Molecule details ›
Chain: S18
Length: 65 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS19 Chain: S19
Molecule details ›
Chain: S19
Length: 79 amino acids
Theoretical weight: 9.06 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS20 Chain: S20
Molecule details ›
Chain: S20
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS21 Chain: S21
Molecule details ›
Chain: S21
Length: 65 amino acids
Theoretical weight: 7.76 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL1 Chain: L1
Molecule details ›
Chain: L1
Length: 223 amino acids
Theoretical weight: 23.74 KDa
Source organism: Escherichia coli
PR20, ALS/FTD dipeptide repeat protein Chain: PR
Molecule details ›
Chain: PR
Length: 40 amino acids
Theoretical weight: 5.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
16S ribosomal RNA Chain: 16S
Molecule details ›
Chain: 16S
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Source organism: Escherichia coli
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: 23S
Molecule details ›
Chain: 23S
Length: 2903 nucleotides
Theoretical weight: 941.31 KDa
Source organism: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: 5S
Molecule details ›
Chain: 5S
Length: 120 nucleotides
Theoretical weight: 38.81 KDa
Source organism: Escherichia coli
Sequence domains: 5S ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-26037
Expression system: Not provided

Quick links

Citations

5 review citations

ALS' Perfect Storm: C9orf72-Associated Toxic Dipeptide Repeats as Potential Multipotent Disruptors of Protein Homeostasis.
Smeele et al. (2024)
4 more