› 7tr2

Electron Microscopy
3Å resolution

Apo CaKip3[2-436]-L2-mutant(HsKHC) in complex with a microtubule

Released:
DOI: 10.2210/pdb7tr2/pdb
PDB entry 7tr2 coloured by chain, front view.
PDB entry 7tr2 coloured by chain, side view.
PDB entry 7tr2 coloured by chain, top view.
Source organisms:
Primary publication:
Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.
Hunter B, Benoit MPMH, Asenjo AB, Doubleday C, Trofimova D, Frazer C, Shoukat I, Sosa H, Allingham JS
OpenAccess logo Nat Commun 13 4198 (2022)
PMID: 35859148
Related structures: EMD-26079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-504154 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-1 heavy chain; Kinesin-like protein Chain: K
Molecule details ›
Chain: K
Length: 420 amino acids
Theoretical weight: 46.34 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: C4YNU9 (Residues: 2-141, 145-436; Coverage: 42%)
  • Canonical: P33176 (Residues: 42-44; Coverage: 0%)
Gene names: CAWG_02882, KIF5B, KNS, KNS1
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-26079
Expression system: Escherichia coli

Quick links

Citations

1 review citation

New insights into the mechanochemical coupling mechanism of kinesin-microtubule complexes from their high-resolution structures.
Benoit et al. (2023)
4 other articles

1 mention without citation

Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.
Hunter et al. (2022)