› 7tt0

Electron Microscopy
4.3Å resolution

BamABCDE bound to substrate EspP class 2

Released:
DOI: 10.2210/pdb7tt0/pdb
PDB entry 7tt0 coloured by chain, front view.
PDB entry 7tt0 coloured by chain, side view.
PDB entry 7tt0 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle MT, Jimah JR, Dowdy T, Ohlemacher SI, Larion M, Hinshaw JE, Bernstein HD
Cell 185 1143-1156.e13 (2022)
PMID: 35294859
Related structures: EMD-26106

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-128462 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 89.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 22-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A937 (Residues: 20-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Outer membrane protein assembly factor BamD Chain: D
Outer membrane protein assembly factor BamC Chain: C
Autotransporter protein EspP translocator; Maltose/maltodextrin-binding periplasmic protein Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26106
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Surveying membrane landscapes: a new look at the bacterial cell surface.
Lithgow et al. (2023)
4 more

1 mention without citation

Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle et al. (2022)