› 7tt5

Electron Microscopy
4.3Å resolution

BamABCDE bound to substrate EspP in the open-sheet EspP state

Released:
DOI: 10.2210/pdb7tt5/pdb
PDB entry 7tt5 coloured by chain, front view.
PDB entry 7tt5 coloured by chain, side view.
PDB entry 7tt5 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle MT, Jimah JR, Dowdy T, Ohlemacher SI, Larion M, Hinshaw JE, Bernstein HD
Cell 185 1143-1156.e13 (2022)
PMID: 35294859
Related structures: EMD-26111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-128462 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Autotransporter protein EspP translocator; Maltose/maltodextrin-binding periplasmic protein Chain: P
Molecule details ›
Chain: P
Length: 764 amino acids
Theoretical weight: 83.43 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 26-392; Coverage: 99%)
  • Canonical: O32591 (Residues: 948-984, 985-1298; Coverage: 28%)
Gene names: JW3994, b4034, espP, malE, pssA
Sequence domains:
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 89.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 22-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamE Chain: E
Outer membrane protein assembly factor BamD Chain: D
Outer membrane protein assembly factor BamC Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26111
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Surveying membrane landscapes: a new look at the bacterial cell surface.
Lithgow et al. (2023)
4 more

2 mentions without citation

Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle et al. (2022)
1 more