› 7tt6

Electron Microscopy
4.3Å resolution

BamABCDE bound to substrate EspP in the intermediate-open EspP state

Released:
DOI: 10.2210/pdb7tt6/pdb
PDB entry 7tt6 coloured by chain, front view.
PDB entry 7tt6 coloured by chain, side view.
PDB entry 7tt6 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle MT, Jimah JR, Dowdy T, Ohlemacher SI, Larion M, Hinshaw JE, Bernstein HD
Cell 185 1143-1156.e13 (2022)
PMID: 35294859
Related structures: EMD-26112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-128462 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Autotransporter protein EspP translocator; Maltose/maltodextrin-binding periplasmic protein Chain: P
Molecule details ›
Chain: P
Length: 764 amino acids
Theoretical weight: 83.43 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 26-392; Coverage: 99%)
  • Canonical: O32591 (Residues: 948-984, 985-1298; Coverage: 28%)
Gene names: JW3994, b4034, espP, malE, pssA
Sequence domains:
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 89.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 22-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A903 (Residues: 25-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 25.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC02 (Residues: 20-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 20-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26112
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

3 review citations

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment.
Krishnarjuna et al. (2022)
2 more

1 mention without citation

Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle et al. (2022)