› 7tuf

X-ray diffraction
2.8Å resolution

Crystal structure of Tapasin in complex with PaSta1-Fab

Released:
DOI: 10.2210/pdb7tuf/pdb
PDB entry 7tuf coloured by chain, front view.
PDB entry 7tuf coloured by chain, side view.
PDB entry 7tuf coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of tapasin-mediated MHC-I peptide loading in antigen presentation.
Jiang J, Taylor DK, Kim EJ, Boyd LF, Ahmad J, Mage MG, Truong HV, Woodward CH, Sgourakis NG, Cresswell P, Margulies DH, Natarajan K
OpenAccess logo Nat Commun 13 5470 (2022)
PMID: 36115831

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224882 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tapasin Chains: C, D
Molecule details ›
Chains: C, D
Length: 416 amino acids
Theoretical weight: 44.74 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O15533 (Residues: 21-401; Coverage: 89%)
  • Best match: O15533-2 (Residues: 21-401)
Gene names: NGS17, TAPA, TAPBP
Sequence domains: Immunoglobulin C1-set domain
PaSta1 Fab heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 233 amino acids
Theoretical weight: 25.5 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
PaSta1 Fab kappa light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 213 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 117.422Å b: 68.988Å c: 142.981Å
α: 90° β: 108.415° γ: 90°
R-values:
R R work R free
0.233 0.231 0.274
Expression systems:
  • Drosophila melanogaster
  • Homo sapiens

Quick links

Citations

4 review citations

Pathways of MHC I cross-presentation of exogenous antigens.
Cruz et al. (2023)
3 more

2 mentions without citation

Chaperone-mediated MHC-I peptide exchange in antigen presentation.
Jiang et al. (2024)
1 more