› 7tuv

X-ray diffraction
2.22Å resolution

Crystal structure of the exoribonucleolytic module of T. brucei RRP44

Released:
DOI: 10.2210/pdb7tuv/pdb
PDB entry 7tuv coloured by chain, front view.
PDB entry 7tuv coloured by chain, side view.
PDB entry 7tuv coloured by chain, top view.
Source organisms:
Primary publication:
Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates.
Cesaro G, da Soler HT, Guerra-Slompo EP, Haouz A, Legrand P, Zanchin NIT, Guimaraes BG
OpenAccess logo Nucleic Acids Res (2022)
PMID: 36583334

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188285 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease II/R domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 745 amino acids
Theoretical weight: 84.36 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95Z12 (Residues: 232-968; Coverage: 76%)
Gene name: RRP44
Sequence domains:
RNA (5'-R(P*GP*GP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.26 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand U5P 1 x U5P
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 66.296Å b: 91.113Å c: 148.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

HELZ2: a new, interferon-regulated, human 3'-5' exoribonuclease of the RNB family is expressed from a non-canonical initiation codon.
Huntzinger et al. (2023)
1 more

1 mention without citation

Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates.
Cesaro et al. (2023)