› 7tv0

X-ray diffraction
2.6Å resolution

Crystal structure of BRD4 bromodomain 1 in complex with dual-acetylated SARS-CoV-2 E

Released:
DOI: 10.2210/pdb7tv0/pdb
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PDB entry 7tv0 coloured by chain, front view.
PDB entry 7tv0 coloured by chain, side view.
PDB entry 7tv0 coloured by chain, top view.
Source organisms:
Primary publication:
Binding of the SARS-CoV-2 envelope E protein to human BRD4 is essential for infection.
Vann KR, Acharya A, Jang SM, Lachance C, Zandian M, Holt TA, Smith AL, Pandey K, Durden DL, El-Gamal D, Côté J, Byrareddy SN, Kutateladze TG
OpenAccess logo Structure (2022)
PMID: 35716662

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-130115 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain-containing protein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 16.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 42-180; Coverage: 10%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Envelope small membrane protein Chains: E, G
Molecule details ›
Chains: E, G
Length: 12 amino acids
Theoretical weight: 1.57 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Not provided
UniProt:
  • Canonical: P0DTC4 (Residues: 53-64; Coverage: 16%)
Gene names: 4, E

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 4 x ALY

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 49.32Å b: 96.22Å c: 78.95Å
α: 90° β: 94.37° γ: 90°
R-values:
R R work R free
0.247 0.243 0.292
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

5 review citations

Protein post-translational modification in SARS-CoV-2 and host interaction.
Cheng et al. (2022)
4 more

3 mentions without citation

Catching BETs by viruses.
Zandian et al. (2022)
2 more

PDB-REDO

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