› 7txv

Electron Microscopy
2.7Å resolution

Cyanophycin synthetase 1 from Synechocystis sp. UTEX2470 E82Q with ATP and 16x(Asp-Arg)

Released:
DOI: 10.2210/pdb7txv/pdb
PDB entry 7txv coloured by chain, front view.
PDB entry 7txv coloured by chain, side view.
PDB entry 7txv coloured by chain, top view.
Source organisms:
Primary publication:
A cryptic third active site in cyanophycin synthetase creates primers for polymerization
Sharon I, Pinus S, Grogg M, Moitessier N, Hilvert D, Schmeing TM
Nat Commun 13 3923- (2022)
Related structures: EMD-26161

Function and Biology Details

Reactions catalysed: 
ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp
ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100400 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyanophycin synthetase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 879 amino acids
Theoretical weight: 95.76 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A068N621 (Residues: 1-873; Coverage: 100%)
Gene names: D082_30240, cphA
Sequence domains:
16x(Asp-Arg) Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 16 amino acids
Theoretical weight: 4.36 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 16 x MG
Ligand ZN 4 x ZN
Ligand ATP 8 x ATP
1 modified residue:
Ligand 7ID 128 x 7ID

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-26161
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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