› 7tyh

Electron Microscopy
3.3Å resolution

Human Amylin2 Receptor in complex with Gs and human calcitonin peptide

Released:
DOI: 10.2210/pdb7tyh/pdb
PDB entry 7tyh coloured by chain, front view.
PDB entry 7tyh coloured by chain, side view.
PDB entry 7tyh coloured by chain, top view.
Source organisms:
Primary publication:
A structural basis for amylin receptor phenotype.
Cao J, Belousoff MJ, Liang YL, Johnson RM, Josephs TM, Fletcher MM, Christopoulos A, Hay DL, Danev R, Wootten D, Sexton PM
Science 375 eabm9609 (2022)
PMID: 35324283
Related structures: EMD-26179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208115 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Receptor activity-modifying protein 2 Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 17.84 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60895 (Residues: 44-175; Coverage: 99%)
Gene name: RAMP2
Sequence domains: Receptor activity modifying family
Calcitonin Chain: P
Molecule details ›
Chain: P
Length: 33 amino acids
Theoretical weight: 3.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01258 (Residues: 85-116; Coverage: 28%)
Gene names: CALC1, CALCA
Calcitonin receptor Chain: R
Molecule details ›
Chain: R
Length: 501 amino acids
Theoretical weight: 58.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30988 (Residues: 25-474; Coverage: 100%)
  • Best match: P30988-3 (Residues: 1-427)
Gene name: CALCR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-26179
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Targeting VIP and PACAP Receptor Signaling: New Insights into Designing Drugs for the PACAP Subfamily of Receptors.
Lu et al. (2022)
4 more

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more