› 7tz1

X-ray diffraction
2.79Å resolution

Crystal Structure of a Mycobacteriophage Cluster A2 Immunity Repressor:DNA Complex

Released:
DOI: 10.2210/pdb7tz1/pdb
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PDB entry 7tz1 coloured by chain, front view.
PDB entry 7tz1 coloured by chain, side view.
PDB entry 7tz1 coloured by chain, top view.
Source organism: Mycobacterium phage TipsytheTRex
Primary publication:
A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
OpenAccess logo Nat Commun 13 4105 (2022)
PMID: 35835745

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104192 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Immunity repressor Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.92 KDa
Source organism: Mycobacterium phage TipsytheTRex
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2D1GKF7 (Residues: 1-183; Coverage: 100%)
Gene name: SEA_TIPSYTHETREX_75
DNA (5'-D(P*TP*TP*TP*CP*GP*GP*TP*GP*GP*CP*TP*GP*TP*CP*AP*AP*GP*CP*GP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
Source organism: Mycobacterium phage TipsytheTRex
Expression system: Not provided
DNA (5'-D(P*CP*CP*CP*GP*CP*TP*TP*GP*AP*CP*AP*GP*CP*CP*AP*CP*CP*GP*AP*AP*A)-3') Chain: C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 131.267Å b: 44.17Å c: 89.448Å
α: 90° β: 102.9° γ: 90°
R-values:
R R work R free
0.203 0.199 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

1 review citation

Using AlphaFold Predictions in Viral Research.
Gutnik et al. (2023)
3 other articles

1 mention without citation

A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.
McGinnis et al. (2022)

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