› 7u22

X-ray diffraction
3.87Å resolution

Mycobacterium tuberculosis RNA polymerase sigma A holoenzyme open promoter complex containing UMN-7

Released:
DOI: 10.2210/pdb7u22/pdb
PDB entry 7u22 coloured by chain, front view.
PDB entry 7u22 coloured by chain, side view.
PDB entry 7u22 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Redesign of Rifamycin Antibiotics to Overcome ADP-Ribosylation-Mediated Resistance.
Lan T, Ganapathy US, Sharma S, Ahn YM, Zimmerman M, Molodtsov V, Hegde P, Gengenbacher M, Ebright RH, Dartois V, Freundlich JS, Dick T, Aldrich CC
OpenAccess logo Angew Chem Int Ed Engl e202211498 (2022)
PMID: 36222275

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-512113 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.75 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycobacterium tuberculosis
UniProt:
  • Canonical: P9WGZ1 (Residues: 1-347; Coverage: 100%)
Gene names: MTCY13E12.10c, Rv3457c, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1178 amino acids
Theoretical weight: 130.02 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycobacterium tuberculosis
UniProt:
  • Canonical: P9WGY9 (Residues: 1-1178; Coverage: 100%)
Gene names: MTCI376.08c, Rv0667, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1316 amino acids
Theoretical weight: 146.97 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycobacterium tuberculosis
UniProt:
  • Canonical: P9WGY7 (Residues: 1-1316; Coverage: 100%)
Gene names: MTCI376.07c, Rv0668, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 110 amino acids
Theoretical weight: 11.85 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycobacterium tuberculosis
UniProt:
  • Canonical: P9WGY5 (Residues: 1-110; Coverage: 100%)
Gene names: MTCY21B4.07, Rv1390, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 528 amino acids
Theoretical weight: 57.88 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycobacterium tuberculosis
UniProt:
  • Canonical: P9WGI1 (Residues: 1-528; Coverage: 100%)
Gene names: MTCY05A6.24, Rv2703, mysA, rpoD, rpoV, sigA
Sequence domains:
T DNA Chain: G
Molecule details ›
Chain: G
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
NT DNA Chain: H
Molecule details ›
Chain: H
Length: 23 nucleotides
Theoretical weight: 7.16 KDa

Ligands and Environments

3 bound ligands:
Ligand KYO 1 x KYO
Ligand ZN 2 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 149.482Å b: 161.445Å c: 196.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.278
Expression system: Mycobacterium tuberculosis

Quick links

Citations

1 review citation

Innovative Phospholipid Carriers: A Viable Strategy to Counteract Antimicrobial Resistance.
Nicolosi et al. (2023)
4 other articles