› 7u32

Electron Microscopy
3.46Å resolution

MVV cleaved synaptic complex (CSC) intasome at 3.4 A resolution

Released:
DOI: 10.2210/pdb7u32/pdb
PDB entry 7u32 coloured by chain, front view.
PDB entry 7u32 coloured by chain, side view.
PDB entry 7u32 coloured by chain, top view.
Source organisms:
Primary publication:
Multivalent interactions essential for lentiviral integrase function.
Ballandras-Colas A, Chivukula V, Gruszka DT, Shan Z, Singh PK, Pye VE, McLean RK, Bedwell GJ, Li W, Nans A, Cook NJ, Fadel HJ, Poeschla EM, Griffiths DJ, Vargas J, Taylor IA, Lyumkis D, Yardimci H, Engelman AN, Cherepanov P
OpenAccess logo Nat Commun 13 2416 (2022)
PMID: 35504909
Related structures: EMD-26322

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endonucleolytic cleavage to 5'-phosphomonoester.
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122117 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Integrase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 281 amino acids
Theoretical weight: 32.37 KDa
Source organism: Visna/maedi virus EV1 KV1772
Expression system: Escherichia coli
UniProt:
  • Canonical: P35956 (Residues: 1226-1506; Coverage: 19%)
Gene name: pol
Sequence domains:
DNA EV273 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 29 nucleotides
Theoretical weight: 8.94 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA EV272 Chains: W, Z
Molecule details ›
Chains: W, Z
Length: 27 nucleotides
Theoretical weight: 8.27 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 12 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-26322
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 citation in other articles

Allosteric Integrase Inhibitor Influences on HIV-1 Integration and Roles of LEDGF/p75 and HDGFL2 Host Factors.
Singh et al. (2022)
5 more

1 mention without citation

Multivalent interactions essential for lentiviral integrase function.
Ballandras-Colas et al. (2022)