Assemblies
Assembly Name:
DNA polymerase alpha:primase complex and DNA
Multimeric state:
hetero octamer
Accessible surface area:
189502.01 Å2
Buried surface area:
21429.1 Å2
Dissociation area:
1,239.93
Å2
Dissociation energy (ΔGdiss):
-15.18
kcal/mol
Dissociation entropy (TΔSdiss):
25.27
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-122144
Macromolecules
Chain: A
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam: DNA primase small subunit
InterPro:
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P49642 (Residues: 1-420; Coverage: 100%)
Pfam: DNA primase small subunit
InterPro:
PDBe-KB: UniProt Coverage View:
P49642
P49642
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Chain: B
Length: 512 amino acids
Theoretical weight: 59.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam: Eukaryotic and archaeal DNA primase, large subunit
InterPro:
Length: 512 amino acids
Theoretical weight: 59.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Pfam: Eukaryotic and archaeal DNA primase, large subunit
InterPro:
PDBe-KB: UniProt Coverage View:
P49643
P49643
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Chain: C
Length: 1132 amino acids
Theoretical weight: 129.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam:
Length: 1132 amino acids
Theoretical weight: 129.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P09884 (Residues: 335-1462; Coverage: 77%)
Pfam:
- DNA polymerase family B, exonuclease domain
- DNA polymerase family B
- DNA Polymerase alpha zinc finger
- DNA-directed DNA polymerase, family B, exonuclease domain
- Ribonuclease H-like superfamily
- DNA-directed DNA polymerase, family B
- Ribonuclease H superfamily
- DNA-directed DNA polymerase, family B, multifunctional domain
- DNA/RNA polymerase superfamily
- DNA polymerase, palm domain superfamily
- DNA polymerase alpha catalytic subunit, catalytic domain
- DNA-directed DNA polymerase, family B, conserved site
- DNA polymerase family B, thumb domain
- DNA polymerase alpha, zinc finger domain superfamily
- Zinc finger, DNA-directed DNA polymerase, family B, alpha
PDBe-KB: UniProt Coverage View:
P09884
P09884
Chains
Domains
Secondary structure
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Chain: D
Length: 598 amino acids
Theoretical weight: 66.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam:
Length: 598 amino acids
Theoretical weight: 66.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q14181 (Residues: 1-598; Coverage: 100%)
Pfam:
- DNA polymerase alpha subunit B N-terminal
- DNA polymerase alpha subunit B, OB domain
- DNA polymerase alpha/epsilon subunit B
PDBe-KB: UniProt Coverage View:
Q14181
Q14181
Chains
Domains
Secondary structure
Flexibility predictions
Interaction interfaces
Sequence conservation
Variants
Chain: E
Length: 1221 amino acids
Theoretical weight: 135.05 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam: CST, telomere maintenance, complex subunit CTC1
InterPro:
Length: 1221 amino acids
Theoretical weight: 135.05 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q2NKJ3 (Residues: 1-1217; Coverage: 100%)
Pfam: CST, telomere maintenance, complex subunit CTC1
InterPro:
PDBe-KB: UniProt Coverage View:
Q2NKJ3
Q2NKJ3
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Chain: F
Length: 368 amino acids
Theoretical weight: 42.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam:
InterPro:
Length: 368 amino acids
Theoretical weight: 42.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q9H668 (Residues: 1-368; Coverage: 100%)
Pfam:
InterPro:
- CST complex subunit Stn1
- Replication factor A protein-like
- Nucleic acid-binding, OB-fold
- OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
- Winged helix DNA-binding domain superfamily
- CST complex subunit Stn1, wHTH1 motif superfamily
- Stn1, C-terminal
- Winged helix-like DNA-binding domain superfamily
PDBe-KB: UniProt Coverage View:
Q9H668
Q9H668
Chains
Domains
Secondary structure
Interaction interfaces
Sequence conservation
Variants
Chain: G
Length: 123 amino acids
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Pfam: Telomere-capping, CST complex subunit
InterPro:
Length: 123 amino acids
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q86WV5 (Residues: 1-123; Coverage: 100%)
Pfam: Telomere-capping, CST complex subunit
InterPro:
PDBe-KB: UniProt Coverage View:
Q86WV5
Q86WV5
Chains
Domains
Secondary structure
Interaction interfaces
Sequence conservation
Variants
Name:
canonical telomeric DNA sequence
Representative chains: H
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
Representative chains: H
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
Chain H (auth H)
Interaction interfaces