Structure analysis

Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state

Electron Microscopy
4.6Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero octamer (preferred)
Entry contents: 7 distinct polypeptide molecules
1 distinct DNA molecule

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero octamer
Accessible surface area: 189502.01 Å2
Buried surface area: 21429.1 Å2
Dissociation area: 1,239.93 Å2
Dissociation energy (ΔGdiss): -15.18 kcal/mol
Dissociation entropy (TΔSdiss): 25.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-122144

Macromolecules

Chain: A
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49642 (Residues: 1-420; Coverage: 100%)
Gene name: PRIM1
Pfam: DNA primase small subunit
InterPro:
PDBe-KB: UniProt Coverage View: P49642  
142050100150200250300350400
 
100200300400
UniProt
P49642
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

Chain: B
Length: 512 amino acids
Theoretical weight: 59.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Gene names: PRIM2, PRIM2A
Pfam: Eukaryotic and archaeal DNA primase, large subunit
InterPro:
PDBe-KB: UniProt Coverage View: P49643  
151250100150200250300350400450500
 
200400
UniProt
P49643
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

PDBe-KB: UniProt Coverage View: P09884  
111321002003004005006007008009001,0001,100
 
5001000
UniProt
P09884
Chains
Domains
Secondary structure
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

PDBe-KB: UniProt Coverage View: Q14181  
159850100150200250300350400450500550
 
200400
UniProt
Q14181
Chains
Domains
Secondary structure
Flexibility predictions
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

Chain: E
Length: 1221 amino acids
Theoretical weight: 135.05 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q2NKJ3 (Residues: 1-1217; Coverage: 100%)
Gene names: C17orf68, CTC1
Pfam: CST, telomere maintenance, complex subunit CTC1
InterPro:
PDBe-KB: UniProt Coverage View: Q2NKJ3  
112211002003004005006007008009001,0001,1001,200
 
5001000
UniProt
Q2NKJ3
Chains
Domains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

PDBe-KB: UniProt Coverage View: Q9H668  
136850100150200250300350
 
100200300
UniProt
Q9H668
Chains
Domains
Secondary structure
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

Chain: G
Length: 123 amino acids
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86WV5 (Residues: 1-123; Coverage: 100%)
Gene names: C17orf106, TEN1
Pfam: Telomere-capping, CST complex subunit
InterPro:
PDBe-KB: UniProt Coverage View: Q86WV5  
1123102030405060708090100110120
 
50100
UniProt
Q86WV5
Chains
Domains
Secondary structure
Interaction interfaces
Sequence conservation
Variants

Search similar proteins

Name: canonical telomeric DNA sequence
Representative chains: H
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
11824681012141618
 
51015GGTTAGGGTTAGGGTTAG
Chains
Chain H (auth H)
Interaction interfaces

Search similar DNA