› 7u5f

X-ray diffraction
2Å resolution

Crystal structure of guanylate kinase from Pseudomonas aeruginosa PAO1 in complex with GMP

Released:
DOI: 10.2210/pdb7u5f/pdb
PDB entry 7u5f coloured by chain, front view.
PDB entry 7u5f coloured by chain, side view.
PDB entry 7u5f coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-574696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 210 amino acids
Theoretical weight: 24.02 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HTM2 (Residues: 1-203; Coverage: 100%)
Gene names: PA5336, gmk
Sequence domains: Guanylate kinase

Ligands and Environments

2 bound ligands:
Ligand 5GP 4 x 5GP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 46.5Å b: 122.9Å c: 149.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.227
Expression system: Escherichia coli BL21(DE3)

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