› 7u6e

Electron Microscopy
3Å resolution

Head region of insulin receptor ectodomain (A-isoform) bound to the non-insulin agonist IM462

Released:
DOI: 10.2210/pdb7u6e/pdb
PDB entry 7u6e coloured by chain, front view.
PDB entry 7u6e coloured by chain, side view.
PDB entry 7u6e coloured by chain, top view.
Source organisms:
Primary publication:
Activation of the human insulin receptor by non-insulin-related peptides.
Kirk NS, Chen Q, Wu YG, Asante AL, Hu H, Espinosa JF, Martínez-Olid F, Margetts MB, Mohammed FA, Kiselyov VV, Barrett DG, Lawrence MC
OpenAccess logo Nat Commun 13 5695 (2022)
PMID: 36171189
Related structures: EMD-26364

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134189 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
Insulin receptor subunit alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 930 amino acids
Theoretical weight: 106.73 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-955; Coverage: 66%)
  • Best match: P06213-2 (Residues: 28-943)
Gene name: INSR
Sequence domains:
IM462 Chains: G, H
Molecule details ›
Chains: G, H
Length: 23 amino acids
Theoretical weight: 2.64 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 2 x NAG
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-26364
Expression systems:
  • Saccharomyces cerevisiae
  • Cricetulus griseus
  • Not provided

Quick links

Citations

1 review citation

A stepwise activation model for the insulin receptor.
Yunn et al. (2023)
2 other articles