› 7u8o

Electron Microscopy
3.5Å resolution

Structure of porcine V-ATPase with mEAK7 and SidK, Rotary state 2

Released:
DOI: 10.2210/pdb7u8o/pdb
PDB entry 7u8o coloured by chain, front view.
PDB entry 7u8o coloured by chain, side view.
PDB entry 7u8o coloured by chain, top view.
Source organisms:
Primary publication:
CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
OpenAccess logo Life Sci Alliance 5 (2022)
PMID: 35794005
Related structures: EMD-26385

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-236680 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
V-type proton ATPase catalytic subunit A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 617 amino acids
Theoretical weight: 68.39 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q29048 (Residues: 1-617; Coverage: 100%)
Gene names: ATP6A1, ATP6V1A, ATP6V1A1
Sequence domains:
Vacuolar proton pump subunit B Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 515 amino acids
Theoretical weight: 57.16 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1W9K3 (Residues: 1-515; Coverage: 100%)
Sequence domains:
V-type proton ATPase subunit C Chain: G
Molecule details ›
Chain: G
Length: 382 amino acids
Theoretical weight: 44.07 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AXI2 (Residues: 1-382; Coverage: 96%)
Gene name: ATP6V1C1
Sequence domains: V-ATPase subunit C
V-type proton ATPase subunit D Chain: H
Molecule details ›
Chain: H
Length: 247 amino acids
Theoretical weight: 28.3 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZJL5 (Residues: 1-247; Coverage: 100%)
Gene name: ATP6V1D
Sequence domains: ATP synthase subunit D
V-type proton ATPase subunit E 1 Chains: I, J, K
Molecule details ›
Chains: I, J, K
Length: 226 amino acids
Theoretical weight: 26.16 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: K9J4U3 (Residues: 1-226; Coverage: 100%)
Gene name: ATP6V1E1
Sequence domains: ATP synthase (E/31 kDa) subunit
V-type proton ATPase subunit F Chain: L
Molecule details ›
Chain: L
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1H0W9 (Residues: 1-119; Coverage: 100%)
Gene name: ATP6V1F
Sequence domains: ATP synthase (F/14-kDa) subunit
V-type proton ATPase subunit G Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 118 amino acids
Theoretical weight: 13.75 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1UZN2 (Residues: 1-118; Coverage: 100%)
Gene names: ATP6V1G1, TMEM268
Sequence domains: Vacuolar (H+)-ATPase G subunit
Type IV secretion protein Dot Chains: Q, R, S
Molecule details ›
Chains: Q, R, S
Length: 337 amino acids
Theoretical weight: 38.54 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZWW6 (Residues: 1-337; Coverage: 59%)
Gene name: lpg0968
V-type proton ATPase subunit H Chain: T
Molecule details ›
Chain: T
Length: 483 amino acids
Theoretical weight: 55.92 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q9TVC1 (Residues: 1-483; Coverage: 100%)
Gene name: ATP6V1H
Sequence domains:
TLDc protein mEAK-7 Chain: U
Molecule details ›
Chain: U
Length: 456 amino acids
Theoretical weight: 50.38 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
V-type proton ATPase subunit a Chain: a
Molecule details ›
Chain: a
Length: 838 amino acids
Theoretical weight: 96.37 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1U3K4 (Residues: 1-838; Coverage: 100%)
Gene name: ATP6V0A1
Sequence domains: V-type ATPase 116kDa subunit family
V-ATPase proteolipid subunit C-like domain-containing protein Chain: b
Molecule details ›
Chain: b
Length: 205 amino acids
Theoretical weight: 21.53 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480L444 (Residues: 1-205; Coverage: 100%)
Sequence domains: ATP synthase subunit C
V-type proton ATPase subunit S1 Chain: c
Molecule details ›
Chain: c
Length: 469 amino acids
Theoretical weight: 51.55 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: K9IWA3 (Residues: 1-469; Coverage: 100%)
Gene name: ATP6AP1
Sequence domains:
V-type proton ATPase subunit Chain: d
Molecule details ›
Chain: d
Length: 351 amino acids
Theoretical weight: 40.37 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1UJS8 (Residues: 1-351; Coverage: 100%)
Sequence domains: ATP synthase (C/AC39) subunit
V-type proton ATPase subunit Chain: e
Molecule details ›
Chain: e
Length: 81 amino acids
Theoretical weight: 9.34 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1U843 (Residues: 1-81; Coverage: 100%)
Gene name: ATP6V0E1
Sequence domains: ATP synthase subunit H
Ribonuclease kappa Chain: f
Molecule details ›
Chain: f
Length: 98 amino acids
Theoretical weight: 11.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480L8C4 (Residues: 118-215; Coverage: 46%)
V-type proton ATPase proteolipid subunit Chains: g, h, i, j, k, l, m, n, o
Molecule details ›
Chains: g, h, i, j, k, l, m, n, o
Length: 155 amino acids
Theoretical weight: 15.64 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VUN6 (Residues: 1-155; Coverage: 100%)
Gene name: ATP6V0C
Sequence domains: ATP synthase subunit C
Renin receptor Chain: p
Molecule details ›
Chain: p
Length: 351 amino acids
Theoretical weight: 39.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AJK1 (Residues: 1-351; Coverage: 100%)
Gene name: ATP6AP2
Sequence domains: Renin receptor-like protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-26385
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Identification of mEAK-7 as a human V-ATPase regulator via cryo-EM data mining.
Wang et al. (2022)
1 more

1 mention without citation

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.
Tan et al. (2022)