› 7u9x

Electron Microscopy
2.58Å resolution

Structure of PKA phosphorylated human RyR2-R2474S in the closed state

Released:
DOI: 10.2210/pdb7u9x/pdb
PDB entry 7u9x coloured by chain, front view.
PDB entry 7u9x coloured by chain, side view.
PDB entry 7u9x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment.
Miotto MC, Weninger G, Dridi H, Yuan Q, Liu Y, Wronska A, Melville Z, Sittenfeld L, Reiken S, Marks AR
OpenAccess logo Sci Adv 8 eabo1272 (2022)
PMID: 35857850
Related structures: EMD-26409

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159297 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ryanodine receptor 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 4967 amino acids
Theoretical weight: 565.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92736 (Residues: 1-4967; Coverage: 100%)
  • Best match: Q92736-2 (Residues: 1-4975)
Gene name: RYR2
Sequence domains:
Peptidyl-prolyl cis-trans isomerase FKBP1B Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 108 amino acids
Theoretical weight: 11.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68106 (Residues: 1-108; Coverage: 100%)
Gene names: FKBP12.6, FKBP1B, FKBP1L, FKBP9, OTK4
Sequence domains: FKBP-type peptidyl-prolyl cis-trans isomerase

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand ATP 8 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.58Å
Relevant EMDB volumes: EMD-26409
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

2 review citations

Mitochondrial Calcium Overload Plays a Causal Role in Oxidative Stress in the Failing Heart.
Dridi et al. (2023)
1 more

3 mentions without citation

Targeting ryanodine receptors to treat human diseases.
Marks AR. (2023)
2 more