› 7ubl

X-ray diffraction
2.18Å resolution

Transcription antitermination factor Qlambda in complex with Q-lambda-binding-element DNA

Released:
DOI: 10.2210/pdb7ubl/pdb
PDB entry 7ubl coloured by chain, front view.
PDB entry 7ubl coloured by chain, side view.
PDB entry 7ubl coloured by chain, top view.
Source organism: Escherichia phage Lambda
Primary publication:
In transcription antitermination by Qλ, NusA induces refolding of Qλ to form a nozzle that extends the RNA polymerase RNA-exit channel.
Yin Z, Bird JG, Kaelber JT, Nickels BE, Ebright RH
Proc Natl Acad Sci U S A 119 e2205278119 (2022)
PMID: 35951650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122181 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitermination protein Q Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.22 KDa
Source organism: Escherichia phage Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03047 (Residues: 62-207; Coverage: 71%)
Gene name: Q
Sequence domains: Antitermination protein
DNA (5'-D(P*CP*AP*CP*CP*CP*AP*AP*TP*TP*TP*TP*AP*TP*TP*CP*AP*AP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 5.74 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided
DNA (5'-D(P*CP*AP*TP*TP*GP*AP*AP*TP*AP*AP*AP*AP*TP*TP*GP*GP*GP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.91 KDa
Source organism: Escherichia phage Lambda
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 131.102Å b: 131.102Å c: 49.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.228
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Kang et al. (2023)
1 more

1 mention without citation

Association between simple sequence repeat-rich chromosome regions and intergenomic translocation breakpoints in natural populations of allopolyploid wild wheats.
Molnár et al. (2011)