› 7ued

X-ray diffraction
3Å resolution

Crystal structure of full length mesothelin bound with MORAb-009 Fab

Released:
DOI: 10.2210/pdb7ued/pdb
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PDB entry 7ued coloured by chain, front view.
PDB entry 7ued coloured by chain, side view.
PDB entry 7ued coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.
Zhan J, Lin D, Watson N, Esser L, Tang WK, Zhang A, Liu X, Hassan R, Gleinich A, Shajahan A, Azadi P, Pastan I, Xia D
OpenAccess logo Cancer Res Commun 3 175-191 (2023)
PMID: 36968141

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233657 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Mesothelin, cleaved form Chain: M
Molecule details ›
Chain: M
Length: 312 amino acids
Theoretical weight: 35.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13421 (Residues: 296-608; Coverage: 51%)
  • Best match: Q13421-2 (Residues: 296-599)
Gene names: MPF, MSLN
Sequence domains: Mesothelin
MORAb-009 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.14 KDa
Source organism: Mus musculus
Expression system: Mus musculus
MORAb-009 Fab heavy chain Chain: H

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I422
Unit cell:
a: 133.447Å b: 133.447Å c: 276.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.237
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus

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Citations

1 review citation

Application of CAR-T cell therapy targeting mesothelin in solid tumor treatment.
Chen et al. (2024)
2 other articles

3 mentions without citation

Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.
Zhan et al. (2023)
2 more

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