› 7ufm

Electron Microscopy
3.9Å resolution

VchTnsC AAA+ with DNA (double heptamer)

Released:
DOI: 10.2210/pdb7ufm/pdb
PDB entry 7ufm coloured by chain, front view.
PDB entry 7ufm coloured by chain, side view.
PDB entry 7ufm coloured by chain, top view.
Source organisms:
Primary publication:
Selective TnsC recruitment enhances the fidelity of RNA-guided transposition.
Hoffmann FT, Kim M, Beh LY, Wang J, Vo PLH, Gelsinger DR, George JT, Acree C, Mohabir JT, Fernández IS, Sternberg SH
Nature (2022)
PMID: 36002573
Related structures: EMD-26477

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-122186 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
VchTnsC Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 311 amino acids
Theoretical weight: 35.07 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
DNA (36-MER) Chain: O
Molecule details ›
Chain: O
Length: 36 nucleotides
Theoretical weight: 11.06 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (36-MER) Chain: P
Molecule details ›
Chain: P
Length: 36 nucleotides
Theoretical weight: 11.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ATP 14 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-26477
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Non-viral chimeric antigen receptor (CAR) T cells going viral.
Balke-Want et al. (2023)
3 more

1 mention without citation

Selective TnsC recruitment enhances the fidelity of RNA-guided transposition.
Hoffmann et al. (2022)