› 7uhk

X-ray diffraction
2.2Å resolution

Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Non-Hydrolyzed Moxalactam (80 ms Snapshot)

Released:
DOI: 10.2210/pdb7uhk/pdb
PDB entry 7uhk coloured by chain, front view.
PDB entry 7uhk coloured by chain, side view.
PDB entry 7uhk coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia K279a
Primary publication:
Time-resolved β-lactam cleavage by L1 metallo-β-lactamase.
Wilamowski M, Sherrell DA, Kim Y, Lavens A, Henning RW, Lazarski K, Shigemoto A, Endres M, Maltseva N, Babnigg G, Burdette SC, Srajer V, Joachimiak A
OpenAccess logo Nat Commun 13 7379 (2022)
PMID: 36450742

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 29.24 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2FTM1 (Residues: 19-290; Coverage: 100%)
Gene name: Smlt2667
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MX0 1 x MX0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P6422
Unit cell:
a: 105.853Å b: 105.853Å c: 99.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.194 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Small-molecule inhibitors of bacterial-producing metallo-β-lactamases: insights into their resistance mechanisms and biochemical analyses of their activities.
Ayipo et al. (2023)
3 other articles