› 7uik

Electron Microscopy
7.7Å resolution

Mediator-PIC Early (Tail A + Upstream DNA & Activator)

Released:
DOI: 10.2210/pdb7uik/pdb
PDB entry 7uik coloured by chain, front view.
PDB entry 7uik coloured by chain, side view.
PDB entry 7uik coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of a transcription pre-initiation complex on a divergent promoter.
Gorbea Colón JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K
Mol Cell (2023)
PMID: 36731470
Related structures: EMD-26547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-122189 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
Regulatory protein GAL4 Chains: T, U
Molecule details ›
Chains: T, U
Length: 147 amino acids
Theoretical weight: 16.9 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P04386 (Residues: 1-147; Coverage: 17%)
Gene names: GAL4, YPL248C
Sequence domains:
Mediator of RNA polymerase II transcription subunit 15 Chain: o
Molecule details ›
Chain: o
Length: 1081 amino acids
Theoretical weight: 120.43 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P19659 (Residues: 1-1081; Coverage: 100%)
Gene names: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W
Sequence domains:
Mediator of RNA polymerase II transcription subunit 2 Chain: b
Molecule details ›
Chain: b
Length: 431 amino acids
Theoretical weight: 47.75 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12124 (Residues: 1-431; Coverage: 100%)
Gene names: D2930, MED2, YDL005C
Sequence domains: Mediator complex subunit 2
Mediator of RNA polymerase II transcription subunit 3 Chain: c
Molecule details ›
Chain: c
Length: 397 amino acids
Theoretical weight: 43.12 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40356 (Residues: 1-397; Coverage: 100%)
Gene names: HRS1, MED3, PGD1, YGL025C
Sequence domains: Mediator complex subunit 3 fungal
Mediator of RNA polymerase II transcription subunit 14 Chain: n
Molecule details ›
Chain: n
Length: 241 amino acids
Theoretical weight: 27.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P19263 (Residues: 834-1074; Coverage: 22%)
Gene names: MED14, RGR1, YLR071C
Mediator of RNA polymerase II transcription subunit 5 Chain: e
Molecule details ›
Chain: e
Length: 1132 amino acids
Theoretical weight: 128.92 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53114 (Residues: 1-1132; Coverage: 100%)
Gene names: G1876, MED5, NUT1, YGL151W
Sequence domains: Mediator complex subunit Med5
Mediator of RNA polymerase II transcription subunit 16 Chain: p
Molecule details ›
Chain: p
Length: 974 amino acids
Theoretical weight: 111.42 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32259 (Residues: 1-974; Coverage: 100%)
Gene names: BEL2, GAL22, MED16, N1135, RYE1, SDI3, SIN4, SSF5, SSN4, TSF3, YNL236W
Sequence domains:
DNA (38-MER) Chain: X
Molecule details ›
Chain: X
Length: 38 nucleotides
Theoretical weight: 11.67 KDa
DNA (37-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 37 nucleotides
Theoretical weight: 11.42 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-26547
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators.
Malik et al. (2023)
2 more

1 mention without citation

Structural basis of a transcription pre-initiation complex on a divergent promoter.
Gorbea Colón et al. (2023)