› 7ula

X-ray diffraction
2.46Å resolution

Structure of the Pseudomonas putida AlgKX modification and secretion complex

Released:
DOI: 10.2210/pdb7ula/pdb
PDB entry 7ula coloured by chain, front view.
PDB entry 7ula coloured by chain, side view.
PDB entry 7ula coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Structure of the AlgKX modification and secretion complex required for alginate production and biofilm attachment in Pseudomonas aeruginosa.
Gheorghita AA, Li YE, Kitova EN, Bui DT, Pfoh R, Low KE, Whitfield GB, Walvoort MTC, Zhang Q, Codée JDC, Klassen JS, Howell PL
OpenAccess logo Nat Commun 13 7631 (2022)
PMID: 36494359

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183500 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alginate biosynthesis protein AlgK Chain: B
Molecule details ›
Chain: B
Length: 461 amino acids
Theoretical weight: 50.42 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88NC7 (Residues: 33-484; Coverage: 100%)
Gene names: PP_1285, algK
Sequence domains: Sel1 repeat
Alginate biosynthesis protein AlgX Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 54.49 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88ND0 (Residues: 1-479; Coverage: 100%)
Gene names: PP_1282, algX
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NI 6 x NI
Ligand CL 1 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: I422
Unit cell:
a: 169.95Å b: 169.95Å c: 143.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.254
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections.
Sanya et al. (2023)
3 more

2 mentions without citation

Pseudomonas aeruginosa AlgF is a protein-protein interaction mediator required for acetylation of the alginate exopolysaccharide.
Low et al. (2023)
1 more