› 7ulc

X-ray diffraction
1.99Å resolution

Crystal structure of queuine salvage enzyme DUF2419 mutant D231N, in complex with queuosine-5'-monophosphate

Released:
DOI: 10.2210/pdb7ulc/pdb
PDB entry 7ulc coloured by chain, front view.
PDB entry 7ulc coloured by chain, side view.
PDB entry 7ulc coloured by chain, top view.
Source organism: Sphaerobacter thermophilus DSM 20745
Primary publication:
Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5'-monophosphate as the biological substrate.
Hung SH, Elliott GI, Ramkumar TR, Burtnyak L, McGrenaghan CJ, Alkuzweny S, Quaiyum S, Iwata-Reuyl D, Pan X, Green BD, Kelly VP, de Crécy-Lagard V, Swairjo MA
OpenAccess logo Nucleic Acids Res (2023)
PMID: 36610787

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504999 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuosine 5'-phosphate N-glycosylase/hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 37.19 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1C7A6 (Residues: 1-323; Coverage: 100%)
Gene names: Sthe_2331, qng1
Sequence domains: Queuosine salvage protein

Ligands and Environments

2 bound ligands:
Ligand 56B 2 x 56B
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 106.254Å b: 106.254Å c: 157.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Queuosine-tRNA promotes sex-dependent learning and memory formation by maintaining codon-biased translation elongation speed.
Cirzi et al. (2023)
2 more