7ulf

X-ray diffraction
1.61Å resolution

l-glutamate/GTP complex of F420-gamma glutamyl ligase (CofE) from Archaeoglobus fulgidus

Released:
Model geometry
Fit model/data

Function and Biology Details

Reactions catalysed:
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-127933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme F420:L-glutamate ligase Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 27.42 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28028 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2256, cofE
Sequence domains: F420-0:Gamma-glutamyl ligase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P4122
Unit cell:
a: 68.71Å b: 68.71Å c: 92.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.212
Expression system: Escherichia coli