Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- RNA polymerase, N-terminal
- DNA-directed RNA polymerase, subunit beta-prime
- RNA polymerase Rpb1, domain 5
- RNA polymerase Rpb1, funnel domain superfamily
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
- DNA-directed RNA polymerase, subunit 2
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-103097 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 54.92 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 464 amino acids
Theoretical weight: 54.92 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
A0A172JIC8 (Residues: 1-464; Coverage: 100%)
DNA-directed RNA polymerase
Molecule details ›
Chain: d
Length: 448 amino acids
Theoretical weight: 51.95 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 448 amino acids
Theoretical weight: 51.95 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: A0A172JIH0 (Residues: 1-426; Coverage: 100%)
DNA-directed RNA polymerase beta subunit
Molecule details ›
Chain: c
Length: 496 amino acids
Theoretical weight: 58.11 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 496 amino acids
Theoretical weight: 58.11 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: A0A172JI16 (Residues: 1-496; Coverage: 100%)
DNA-directed RNA polymerase
Molecule details ›
Chain: D
Length: 631 amino acids
Theoretical weight: 73.1 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: RNA polymerase Rpb1, domain 5
Length: 631 amino acids
Theoretical weight: 73.1 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: A0A172JI62 (Residues: 1-631; Coverage: 100%)
Sequence domains: RNA polymerase Rpb1, domain 5
DNA-directed RNA polymerase
Molecule details ›
Chain: C
Length: 665 amino acids
Theoretical weight: 76.98 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: RNA polymerase Rpb2, domain 6
Length: 665 amino acids
Theoretical weight: 76.98 KDa
Source organism: Bacillus phage AR9
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: A0A172JHZ2 (Residues: 1-665; Coverage: 100%)
Sequence domains: RNA polymerase Rpb2, domain 6
