7une

Electron Microscopy
3.73Å resolution

The V1 region of bovine V-ATPase in complex with human mEAK7 (focused refinement)

Released:
Source organisms:
Primary publication:
Molecular basis of mEAK7-mediated human V-ATPase regulation.
OpenAccess logo Nat Commun 13 3272 (2022)
PMID: 35672408
Related structures: EMD-26622

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-245082 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
V-type proton ATPase catalytic subunit A Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 617 amino acids
Theoretical weight: 68.42 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P31404 (Residues: 1-617; Coverage: 100%)
Gene names: ATP6A1, ATP6V1A, ATP6V1A1
Sequence domains:
V-type proton ATPase subunit D Chain: D
Molecule details ›
Chain: D
Length: 247 amino acids
Theoretical weight: 28.3 KDa
Source organism: Bos taurus
KIAA1609 protein, isoform CRA_a Chain: U
V-type proton ATPase subunit E 1 Chains: b, c, d
V-type proton ATPase subunit B, brain isoform Chains: O, P, Q
V-type proton ATPase subunit G 2 Chains: e, f, g

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.73Å
Relevant EMDB volumes: EMD-26622
Expression system: Escherichia coli