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Electron Microscopy
4.02Å resolution

The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664

Released:
Entry authors: Nguyen DN, Tolbert WD, Pazgier M
Related structures: EMD-26648

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-280196 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (11 distinct):
Envelope glycoprotein gp160 Chains: A, G, I
Molecule details ›
Chains: A, G, I
Length: 481 amino acids
Theoretical weight: 54.06 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-505; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, C, J
Molecule details ›
Chains: B, C, J
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
N49-P9.6-FR3 Fab heavy chain Chains: D, H, K
Molecule details ›
Chains: D, H, K
Length: 230 amino acids
Theoretical weight: 25.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
N49-P9.6-FR3 Fab light chain Chains: E, L, M
Molecule details ›
Chains: E, L, M
Length: 203 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT121 Fab light chain Chains: e, l, m
Molecule details ›
Chains: e, l, m
Length: 213 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT121 Fab heavy chain Chains: d, h, k
Molecule details ›
Chains: d, h, k
Length: 235 amino acids
Theoretical weight: 25.17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.02Å
Relevant EMDB volumes: EMD-26648
Expression system: Homo sapiens