› 7urd

Electron Microscopy
2.92Å resolution

Human PORCN in complex with LGK974 and WNT3A peptide

Released:
DOI: 10.2210/pdb7urd/pdb
PDB entry 7urd coloured by chain, front view.
PDB entry 7urd coloured by chain, side view.
PDB entry 7urd coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms and inhibition of Porcupine-mediated Wnt acylation.
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature 607 816-822 (2022)
PMID: 35831507
Related structures: EMD-26709

Function and Biology Details

Reaction catalysed: 
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-225689 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein-serine O-palmitoleoyltransferase porcupine Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 52.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H237 (Residues: 2-461; Coverage: 99%)
  • Best match: Q9H237-2 (Residues: 2-456)
Gene names: MG61, PORC, PORCN, PPN
Sequence domains: MBOAT, membrane-bound O-acyltransferase family
Protein Wnt-3a Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P56704 (Residues: 199-220; Coverage: 7%)
Gene name: WNT3A
2C11 light chain Chain: L
Molecule details ›
Chain: L
Length: 234 amino acids
Theoretical weight: 25.67 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
2C11 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 250 amino acids
Theoretical weight: 26.93 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

5 bound ligands:
Ligand O50 1 x O50
Ligand CLR 2 x CLR
Ligand AJP 1 x AJP
Ligand OLA 1 x OLA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.92Å
Relevant EMDB volumes: EMD-26709
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

6 review citations

Cellular and Molecular Mechanisms of the Tumor Stroma in Colorectal Cancer: Insights into Disease Progression and Therapeutic Targets.
Shakhpazyan et al. (2023)
5 more

4 mentions without citation

A rising tide lifts all MBOATs: recent progress in structural and functional understanding of membrane bound O-acyltransferases.
Pierce et al. (2023)
3 more