› 7ute

Electron crystallography
2.1Å resolution

MicroED structure of Aeropyrum pernix protoglobin mutant

Released:
DOI: 10.2210/pdb7ute/pdb
PDB entry 7ute coloured by chain, front view.
PDB entry 7ute coloured by chain, side view.
PDB entry 7ute coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Biocatalytic Carbene Transfer Using Diazirines.
Porter NJ, Danelius E, Gonen T, Arnold FH
J Am Chem Soc 144 8892-8896 (2022)
PMID: 35561334
Related structures: EMD-26768

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-sensor domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 195 amino acids
Theoretical weight: 22.72 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YFF4 (Residues: 1-195; Coverage: 100%)
Gene name: APE_0287
Sequence domains: Protoglobin

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.1Å
Relevant EMDB volumes: EMD-26768
Expression system: Escherichia coli

Quick links

Citations

3 review citations

MicroED in drug discovery.
Danelius et al. (2023)
2 more

1 mention without citation

Biocatalytic Carbene Transfer Using Diazirines.
Porter et al. (2022)