› 7uus

Electron Microscopy
8Å resolution

The CryoEM structure of the [NiFe]-hydrogenase Huc from Mycobacterium smegmatis - Full complex focused refinement of stalk

Released:
DOI: 10.2210/pdb7uus/pdb
PDB entry 7uus coloured by chain, front view.
PDB entry 7uus coloured by chain, side view.
PDB entry 7uus coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural basis for bacterial energy extraction from atmospheric hydrogen.
Grinter R, Kropp A, Venugopal H, Senger M, Badley J, Cabotaje PR, Jia R, Duan Z, Huang P, Stripp ST, Barlow CK, Belousoff M, Shafaat HS, Cook GM, Schittenhelm RB, Vincent KA, Khalid S, Berggren G, Greening C
OpenAccess logo Nature (2023)
PMID: 36890228
Related structures: EMD-26802

Function and Biology Details

Reaction catalysed: 
H(2) + A = AH(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-222100 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hydrogenase-2, large subunit Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 515 amino acids
Theoretical weight: 57.45 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QUM7 (Residues: 2-516; Coverage: 96%)
Gene names: MSMEG_2263, hybC
Sequence domains: Nickel-dependent hydrogenase
Hydrogenase-2, small subunit Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 369 amino acids
Theoretical weight: 39.66 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QUM6 (Residues: 2-323; Coverage: 100%)
Gene names: MSMEG_2262, hybA
Sequence domains:
DUF1641 domain-containing protein Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 188 amino acids
Theoretical weight: 19.28 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QUM5 (Residues: 2-189; Coverage: 100%)
Gene name: MSMEG_2261
Sequence domains: Protein of unknown function (DUF1641)

Ligands and Environments

5 bound ligands:
Ligand 3NI 8 x 3NI
Ligand FCO 8 x FCO
Ligand MG 8 x MG
Ligand MQ9 8 x MQ9
Ligand F3S 24 x F3S
1 modified residue:
Ligand DHI 8 x DHI

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-26802

Quick links

Citations

2 review citations

Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis.
Ray et al. (2023)
1 more

3 mentions without citation

Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion.
Greening et al. (2023)
2 more