› 7uwa

Electron Microscopy
4.3Å resolution

Citrus V-ATPase State 1, H in contact with subunits AB

Released:
DOI: 10.2210/pdb7uwa/pdb
PDB entry 7uwa coloured by chain, front view.
PDB entry 7uwa coloured by chain, side view.
PDB entry 7uwa coloured by chain, top view.
Source organism: Citrus x limon
Primary publication:
Structure of V-ATPase from citrus fruit.
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022)
PMID: 36041457
Related structures: EMD-26826

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-100356 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
V-type proton ATPase catalytic subunit A Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 623 amino acids
Theoretical weight: 68.76 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: Q9SM09 (Residues: 1-623; Coverage: 100%)
Sequence domains:
Vacuolar proton pump subunit B Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 488 amino acids
Theoretical weight: 54.42 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A067FXK2 (Residues: 1-488; Coverage: 100%)
Gene name: CISIN_1g011329mg
Sequence domains:
V-type proton ATPase subunit D Chain: M
Molecule details ›
Chain: M
Length: 259 amino acids
Theoretical weight: 29.24 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A067FFQ8 (Residues: 1-259; Coverage: 100%)
Gene name: CISIN_1g025029mg
Sequence domains: ATP synthase subunit D
V-type proton ATPase subunit F Chain: N
Molecule details ›
Chain: N
Length: 130 amino acids
Theoretical weight: 14.3 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A067E4V9 (Residues: 1-130; Coverage: 100%)
Gene name: CISIN_1g037034mg
Sequence domains: ATP synthase (F/14-kDa) subunit
V-type proton ATPase subunit AP1 fragment Chain: b
Molecule details ›
Chain: b
Length: 32 amino acids
Theoretical weight: 2.74 KDa
Source organism: Citrus x limon
V-ATPase proteolipid subunit C-like domain-containing protein Chain: c
Molecule details ›
Chain: c
Length: 182 amino acids
Theoretical weight: 18.58 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: Q84V02 (Residues: 1-182; Coverage: 100%)
Gene name: VMA16
Sequence domains: ATP synthase subunit C
V-type proton ATPase subunit d2 Chain: d
Molecule details ›
Chain: d
Length: 351 amino acids
Theoretical weight: 40.69 KDa
Source organism: Citrus x limon
V-type proton ATPase subunit c1 Chains: g, h, i, j, k, l, m, n, o
Molecule details ›
Chains: g, h, i, j, k, l, m, n, o
Length: 164 amino acids
Theoretical weight: 16.58 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: P0DH92 (Residues: 1-164; Coverage: 100%)
Gene names: AVA-P1, AVAP1, At4g34720, T4L20.300, VHA-c1
Sequence domains: ATP synthase subunit C
V-type proton ATPase subunit AP2 fragment Chain: r
Molecule details ›
Chain: r
Length: 24 amino acids
Theoretical weight: 2.06 KDa
Source organism: Citrus x limon
V-type proton ATPase subunit E Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 230 amino acids
Theoretical weight: 26.33 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: Q9MB46 (Residues: 1-230; Coverage: 100%)
Gene name: VATE
Sequence domains: ATP synthase (E/31 kDa) subunit
V-type proton ATPase subunit G Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 110 amino acids
Theoretical weight: 12.35 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A067DRZ4 (Residues: 1-110; Coverage: 100%)
Gene name: CISIN_1g033870mg
Sequence domains: Vacuolar (H+)-ATPase G subunit
V-type proton ATPase subunit C Chain: O
Molecule details ›
Chain: O
Length: 375 amino acids
Theoretical weight: 42.5 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A2H5Q4L7 (Residues: 1-375; Coverage: 100%)
Gene name: CUMW_192980
Sequence domains: V-ATPase subunit C
V-type proton ATPase subunit a Chain: a
Molecule details ›
Chain: a
Length: 823 amino acids
Theoretical weight: 92.94 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: V4UBK8 (Residues: 1-823; Coverage: 100%)
Gene name: CICLE_v10027828mg
Sequence domains: V-type ATPase 116kDa subunit family
V-type proton ATPase subunit e1 Chain: e
Molecule details ›
Chain: e
Length: 70 amino acids
Theoretical weight: 7.76 KDa
Source organism: Citrus x limon
UniProt:
  • Canonical: A0A067F7P5 (Residues: 1-70; Coverage: 100%)
Gene name: CISIN_1g035231mg
Sequence domains: ATP synthase subunit H
V-type proton ATPase subunit H Chain: P
Molecule details ›
Chain: P
Length: 452 amino acids
Theoretical weight: 51.61 KDa
Source organism: Citrus x limon

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26826

Quick links

Citations

3 review citations

Eat or Be Eaten: Strategies Used by Legionella to Acquire Host-Derived Nutrients and Evade Lysosomal Degradation.
Shames SR. (2023)
2 more