› 7uy2

X-ray diffraction
2.51Å resolution

Structure of RNF31 in complex with FP06649, a Helicon Polypeptide

Released:
DOI: 10.2210/pdb7uy2/pdb
PDB entry 7uy2 coloured by chain, front view.
PDB entry 7uy2 coloured by chain, side view.
PDB entry 7uy2 coloured by chain, top view.
Source organisms:
Primary publication:
De novo mapping of α-helix recognition sites on protein surfaces using unbiased libraries.
Li K, Tokareva OS, Thomson TM, Wahl SCT, Travaline TL, Ramirez JD, Choudary SK, Agarwal S, Walkup WG, Olsen TJ, Brennan MJ, Verdine GL, McGee JH
OpenAccess logo Proc Natl Acad Sci U S A 119 e2210435119 (2022)
PMID: 36534810

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168952 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase RNF31 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EP0 (Residues: 1-179; Coverage: 17%)
Gene names: RNF31, ZIBRA
Sequence domains:
Helicon FP06649 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NH2 2 x NH2
Ligand WHL 2 x WHL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P61
Unit cell:
a: 49.42Å b: 49.42Å c: 267.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.211 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Dystrophin- and Utrophin-Based Therapeutic Approaches for Treatment of Duchenne Muscular Dystrophy: A Comparative Review.
Szwec et al. (2023)
4 other articles