› 7uyn

X-ray diffraction
1.65Å resolution

Crystal structure of B-form alien DNA 5'-CTTBPPBBSSZZSAAG in a host-guest complex with the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase

Released:
DOI: 10.2210/pdb7uyn/pdb
PDB entry 7uyn coloured by chain, front view.
PDB entry 7uyn coloured by chain, side view.
PDB entry 7uyn coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of 'ALternative Isoinformational ENgineered' DNA in B-form.
Shukla MS, Hoshika S, Benner SA, Georgiadis MM
OpenAccess logo Philos Trans R Soc Lond B Biol Sci 378 20220028 (2023)
PMID: 36633282

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-512184 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Reverse transcriptase/ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.02 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 683-937; Coverage: 15%)
Gene name: gag-pol
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
DNA (5'-D(*CP*TP*TP*(IGU)P*(1WA)P*(1WA)P*(IGU)P*(IGU))-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.5 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*(JSP)P*(JSP)P*(1W5)P*(1W5)P*(JSP)P*AP*AP*G)-3') Chain: G
Molecule details ›
Chain: G
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand IGU 3 x IGU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21212
Unit cell:
a: 54.854Å b: 146.48Å c: 46.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Reactivity and mechanism in chemical and synthetic biology.
Richards et al. (2023)

1 mention without citation

Crystal structures of 'ALternative Isoinformational ENgineered' DNA in B-form.
Shukla et al. (2023)