› 7v1k

X-ray diffraction
3.29Å resolution

Apo structure of sNASP core

Released:
DOI: 10.2210/pdb7v1k/pdb
PDB entry 7v1k coloured by chain, front view.
PDB entry 7v1k coloured by chain, side view.
PDB entry 7v1k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes.
Bao H, Carraro M, Flury V, Liu Y, Luo M, Chen L, Groth A, Huang H
OpenAccess logo Nucleic Acids Res 50 5349-5368 (2022)
PMID: 35489058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear autoantigenic sperm protein Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P49321 (Residues: 499-679; Coverage: 23%)
Gene name: NASP
Sequence domains: SHNi-TPR

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6222
Unit cell:
a: 95.358Å b: 95.358Å c: 206.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.239 0.261
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Histone 3.3-related chromatinopathy: missense variants throughout H3-3A and H3-3B cause a range of functional consequences across species.
Bryant et al. (2023)
10 other articles

1 mention without citation

NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes.
Bao et al. (2022)