› 7v3o

X-ray diffraction
1.83Å resolution

The structure of Se-SoBcmB with Fe(II)and AKG

Released:
DOI: 10.2210/pdb7v3o/pdb
PDB entry 7v3o coloured by chain, front view.
PDB entry 7v3o coloured by chain, side view.
PDB entry 7v3o coloured by chain, top view.
Source organism: Streptomyces ossamyceticus
Primary publication:
Enzymatic catalysis favours eight-membered over five-membered ring closure in bicyclomycin biosynthesis
He JB, Wu L, Wei W, Meng S, Liu ZT, Wu X, Pan HX, Yang S, Liang Y, Zhou J, Tang GL
Nat Catal 6 637-648 (2023)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/2OG dependent dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.48 KDa
Source organism: Streptomyces ossamyceticus
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand AKG 1 x AKG
Ligand FE2 1 x FE2
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6122
Unit cell:
a: 101.62Å b: 101.62Å c: 130.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.2 0.227
Expression system: Escherichia coli BL21(DE3)

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