7v3x

X-ray diffraction
3.1Å resolution

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-181274 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Circadian clock oscillator protein KaiC Chains: A, B, G, H, M, N, P, R, S, T, V, W, X
Molecule details ›
Chains: A, B, G, H, M, N, P, R, S, T, V, W, X
Length: 519 amino acids
Theoretical weight: 58.15 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Circadian clock oscillator protein KaiC Chains: C, D, E, F, I, J, K, L, O, Q, U
Molecule details ›
Chains: C, D, E, F, I, J, K, L, O, Q, U
Length: 519 amino acids
Theoretical weight: 58.23 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC

Ligands and Environments

3 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 185.492Å b: 205.803Å c: 186.177Å
α: 90° β: 115.14° γ: 90°
R-values:
R R work R free
0.278 0.274 0.34
Expression system: Escherichia coli BL21(DE3)