› 7v8j

X-ray diffraction
2.52Å resolution

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in complex with the reaction intermediates proline-adenylate (Pro-AMP) and pyrophosphate

Released:
DOI: 10.2210/pdb7v8j/pdb
PDB entry 7v8j coloured by chain, front view.
PDB entry 7v8j coloured by chain, side view.
PDB entry 7v8j coloured by chain, top view.
Source organism: Toxoplasma gondii
Entry authors: Manickam Y, Malhotra N, Sharma A

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proline--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 500 amino acids
Theoretical weight: 57.94 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7J6JUK2 (Residues: 215-711; Coverage: 70%)
Gene name: TGRH88_057780
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PR8 1 x PR8
Ligand PPV 1 x PPV
Ligand EDO 4 x EDO
Ligand PRO 1 x PRO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 77.08Å b: 81.72Å c: 105.26Å
α: 90° β: 102.55° γ: 90°
R-values:
R R work R free
0.172 0.169 0.226
Expression system: Escherichia coli

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