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Cryo-EM structures of LolCDE reveal the molecular mechanism of bacterial lipoprotein sorting in Escherichia coli.

<div class='caption-title'>Cryo-EM structures of LolCDE in different states.</div>
<div class='caption-title'>Verification of the apo-LolCDE structure.</div>
<div class='caption-title'>The bipartite binding mode between RcsF and LolCDE.</div>
Bei W, Luo Q, Shi H, Zhou H, Zhou M, Zhang X, Huang Y
OpenAccess logo PLoS Biol 20 e3001823 (2022)
Related entries: 7v8i, 7v8m

Cited: 5 times
EuropePMC logo PMID: 36228045

Abstract

Bacterial lipoproteins perform a diverse array of functions including bacterial envelope biogenesis and microbe-host interactions. Lipoproteins in gram-negative bacteria are sorted to the outer membrane (OM) via the localization of lipoproteins (Lol) export pathway. The ATP-binding cassette (ABC) transporter LolCDE initiates the Lol pathway by selectively extracting and transporting lipoproteins for trafficking. Here, we report cryo-EM structures of LolCDE in apo, lipoprotein-bound, and AMPPNP-bound states at a resolution of 3.5 to 4.2 Å. Structure-based disulfide crosslinking, photo-crosslinking, and functional complementation assay verify the apo-state structure and reveal the molecular details regarding substrate selectivity and substrate entry route. Our studies snapshot 3 functional states of LolCDE in a transport cycle, providing deep insights into the mechanisms that underlie LolCDE-mediated lipoprotein sorting in E. coli.

Reviews citing this publication (2)

  1. ABC Transporters in Bacterial Nanomachineries. Bilsing FL, Anlauf MT, Hachani E, Khosa S, Schmitt L. Int J Mol Sci 24 6227 (2023)
  2. Envelope-Stress Sensing Mechanism of Rcs and Cpx Signaling Pathways in Gram-Negative Bacteria. Cho SH, Dekoninck K, Collet JF. J Microbiol 61 317-329 (2023)

Articles citing this publication (3)

  1. Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope. Lach SR, Kumar S, Kim S, Im W, Konovalova A. PLoS Genet 19 e1010601 (2023)
  2. Identification of an Essential LolD-Like Protein in Helicobacter pylori. McClain MS, Bryant KN, McDonald WH, Algood HMS, Cover TL. J Bacteriol 205 e0005223 (2023)
  3. Activator-induced conformational changes regulate division-associated peptidoglycan amidases. Cook J, Baverstock TC, McAndrew MBL, Roper DI, Stansfeld PJ, Crow A. Proc Natl Acad Sci U S A 120 e2302580120 (2023)


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