› 7v9l

Electron Microscopy
2.6Å resolution

Cryo-EM structure of the SV1-Gs complex.

Released:
DOI: 10.2210/pdb7v9l/pdb
PDB entry 7v9l coloured by chain, front view.
PDB entry 7v9l coloured by chain, side view.
PDB entry 7v9l coloured by chain, top view.
Source organisms:
Primary publication:
Constitutive signal bias mediated by the human GHRHR splice variant 1.
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34599099
Related structures: EMD-31824

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211304 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 41.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-19, 61-181, 229-242; Coverage: 40%)
  • Canonical: P63092 (Residues: 27-67, 205-251, 266-394; Coverage: 55%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
G-protein coupled receptors family 2 profile 2 domain-containing protein Chain: R
Molecule details ›
Chain: R
Length: 522 amino acids
Theoretical weight: 57.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HB45 (Residues: 1-341; Coverage: 95%)
Gene names: GHRHR, hCG_37325
Sequence domains: 7 transmembrane receptor (Secretin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 140 amino acids
Theoretical weight: 15.34 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-31824
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

CHARMM-GUI Membrane Builder: Past, Current, and Future Developments and Applications.
Feng et al. (2023)
5 other articles

2 mentions without citation

New Insights into the Structure and Function of Class B1 GPCRs.
Cary et al. (2023)
1 more