› 7vbc

Electron Microscopy
3.01Å resolution

Back track state of human RNA Polymerase I Elongation Complex

Released:
DOI: 10.2210/pdb7vbc/pdb
PDB entry 7vbc coloured by chain, front view.
PDB entry 7vbc coloured by chain, side view.
PDB entry 7vbc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human RNA polymerase I elongation complex.
Zhao D, Liu W, Chen K, Wu Z, Yang H, Xu Y
OpenAccess logo Cell Discov 7 97 (2021)
PMID: 34671025
Related structures: EMD-31878

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122220 (preferred)
Entry contents:
13 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (16 distinct):
DNA-directed RNA polymerase I subunit RPA1 Chain: A
Molecule details ›
Chain: A
Length: 1719 amino acids
Theoretical weight: 194.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O95602 (Residues: 1-1719; Coverage: 100%)
Gene name: POLR1A
Sequence domains:
DNA-directed RNA polymerase I subunit RPA2 Chain: B
Molecule details ›
Chain: B
Length: 1135 amino acids
Theoretical weight: 128.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H9Y6 (Residues: 1-1135; Coverage: 100%)
Gene name: POLR1B
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: C
Molecule details ›
Chain: C
Length: 346 amino acids
Theoretical weight: 39.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15160 (Residues: 1-346; Coverage: 100%)
Gene names: POLR1C, POLR1E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
Molecule details ›
Chain: E
Length: 210 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P19388 (Residues: 1-210; Coverage: 100%)
Gene name: POLR2E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61218 (Residues: 1-127; Coverage: 100%)
Gene names: POLR2F, POLRF
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P52434 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
Molecule details ›
Chain: J
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62875 (Residues: 1-67; Coverage: 100%)
Gene name: POLR2L
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: K
Molecule details ›
Chain: K
Length: 133 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DPB6 (Residues: 1-133; Coverage: 100%)
Gene name: POLR1D
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Molecule details ›
Chain: L
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P53803 (Residues: 1-58; Coverage: 100%)
Gene name: POLR2K
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
DNA-directed RNA polymerase I subunit RPA34 Chain: N
Molecule details ›
Chain: N
Length: 510 amino acids
Theoretical weight: 55.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15446 (Residues: 1-510; Coverage: 100%)
  • Best match: O15446-2 (Residues: 1-512)
Gene names: ASE1, CAST, CD3EAP, PAF49, POLR1G
Sequence domains: DNA-directed RNA polymerase I subunit RPA34.5
DNA-directed RNA polymerase I subunit RPA43 Chain: G
Molecule details ›
Chain: G
Length: 338 amino acids
Theoretical weight: 37.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q3B726 (Residues: 1-338; Coverage: 100%)
Gene names: POLR1F, TWISTNB
Sequence domains:
DNA-directed RNA polymerase I subunit RPA49 Chain: M
Molecule details ›
Chain: M
Length: 419 amino acids
Theoretical weight: 47.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9GZS1 (Residues: 1-419; Coverage: 100%)
Gene names: PAF53, POLR1E, PRAF1
Sequence domains: A49-like RNA polymerase I associated factor
DNA-directed RNA polymerase I subunit RPA12 Chain: I
Molecule details ›
Chain: I
Length: 126 amino acids
Theoretical weight: 13.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P1U0 (Residues: 1-126; Coverage: 100%)
Gene names: POLR1H, RPA12, ZNRD1
Sequence domains: Transcription factor S-II (TFIIS)
DNA (5'-D(P*AP*GP*GP*AP*CP*AP*GP*CP*GP*TP*GP*TP*CP*AP*GP*CP*AP*AP*TP*A)-3') Chain: T
Molecule details ›
Chain: T
Length: 20 nucleotides
Theoretical weight: 6.19 KDa
DNA (5'-D(*GP*TP*AP*CP*TP*GP*TP*CP*CP*TP*CP*TP*GP*G)-3') Chain: U
Molecule details ›
Chain: U
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
RNA (5'-R(P*UP*GP*CP*UP*GP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 6 nucleotides
Theoretical weight: 1.89 KDa

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-31878
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Targeting Ribosome Biogenesis in Cancer: Lessons Learned and Way Forward.
Zisi et al. (2022)
5 more