› 7vcq

X-ray diffraction
3Å resolution

structure of viral protein BKRF4 in complex with H3.3-H4-ASF1

Released:
DOI: 10.2210/pdb7vcq/pdb
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PDB entry 7vcq coloured by chain, front view.
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PDB entry 7vcq coloured by chain, top view.
Source organisms:
Primary publication:
Epstein-Barr Virus Tegument Protein BKRF4 is a Histone Chaperone.
Liu Y, Li Y, Bao H, Liu Y, Chen L, Huang H
J Mol Biol 167756 (2022)
PMID: 35870648

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero trimer
PDBe Complex ID:
PDB-CPX-151640 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone H3.3 Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 79 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84243 (Residues: 58-136; Coverage: 58%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 1-103; Coverage: 100%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone chaperone ASF1B Chains: D, F, I
Tegument protein BKRF4 Chain: K

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 286.933Å b: 74.77Å c: 61.191Å
α: 90° β: 97.5° γ: 90°
R-values:
R R work R free
0.247 0.245 0.283
Expression system: Escherichia coli

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Citations

1 review citation

Tegument proteins of Epstein-Barr virus: Diverse functions, complex networks, and oncogenesis.
Murata T. (2023)

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