› 7vfo

X-ray diffraction
3.2Å resolution

Crystal structure of SdgB (Phosphate-binding form)

Released:
DOI: 10.2210/pdb7vfo/pdb
PDB entry 7vfo coloured by chain, front view.
PDB entry 7vfo coloured by chain, side view.
PDB entry 7vfo coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus USA300
Primary publication:
Structural basis for SdgB- and SdgA-mediated glycosylation of staphylococcal adhesive proteins.
Kim DG, Baek I, Lee Y, Kim H, Kim JY, Bang G, Kim S, Yoon HJ, Han BW, Suh SW, Kim HS
OpenAccess logo Acta Crystallogr D Struct Biol 77 1460-1474 (2021)
PMID: 34726173

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl transferase family 1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 504 amino acids
Theoretical weight: 59.89 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2XGN0 (Residues: 1-496; Coverage: 100%)
Gene name: SAUSA300_0550
Sequence domains: Glycosyl transferases group 1

Ligands and Environments

1 bound ligand:
Ligand PO4 11 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P3221
Unit cell:
a: 172.257Å b: 172.257Å c: 106.174Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.262
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Analysis and Verification of Glycosylation Characteristics of Septic Acute Kidney Injury.
Chen et al. (2023)

1 mention without citation

Structural basis for SdgB- and SdgA-mediated glycosylation of staphylococcal adhesive proteins.
Kim et al. (2021)