› 7vh5

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the autoinhibited state (pH 7.4, C1 symmetry)

Released:
DOI: 10.2210/pdb7vh5/pdb
PDB entry 7vh5 coloured by chain, front view.
PDB entry 7vh5 coloured by chain, side view.
PDB entry 7vh5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure and activation mechanism of the hexameric plasma membrane H+-ATPase.
Zhao P, Zhao C, Chen D, Yun C, Li H, Bai L
OpenAccess logo Nat Commun 12 6439 (2021)
PMID: 34750373
Related structures: EMD-31986

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + H(+)(Side 1) = ADP + phosphate + H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma membrane ATPase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 918 amino acids
Theoretical weight: 99.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05030 (Residues: 1-918; Coverage: 100%)
Gene names: PMA1, YGL008C
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand POV 51 x POV
Ligand SPH 1 x SPH
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-31986
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Membrane homeostasis beyond fluidity: control of membrane compressibility.
Renne et al. (2023)
4 more

2 mentions without citation

Fungal Plasma Membrane H+-ATPase: Structure, Mechanism, and Drug Discovery.
Zhao et al. (2024)
1 more