› 7vhp

Electron Microscopy
3.27Å resolution

Structural insights into the membrane microdomain organization by SPFH family proteins

Released:
DOI: 10.2210/pdb7vhp/pdb
PDB entry 7vhp coloured by chain, front view.
PDB entry 7vhp coloured by chain, side view.
PDB entry 7vhp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural insights into the membrane microdomain organization by SPFH family proteins.
Ma C, Wang C, Luo D, Yan L, Yang W, Li N, Gao N
Cell Res 32 176-189 (2022)
PMID: 34975153
Related structures: EMD-32002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
PDBe Complex ID:
PDB-CPX-141986 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent zinc metalloprotease FtsH Chains: A, B, C, D, J, K, L, M, T, V, W, X, Y, Z, a, b, c, d, e, f, s, t, u, v
Molecule details ›
Chains: A, B, C, D, J, K, L, M, T, V, W, X, Y, Z, a, b, c, d, e, f, s, t, u, v
Length: 644 amino acids
Theoretical weight: 70.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AAI3 (Residues: 1-644; Coverage: 100%)
Gene names: JW3145, b3178, ftsH, hflB, mrsC, std, tolZ
Sequence domains:
Modulator of FtsH protease HflK Chains: E, F, N, O, S, U, g, h, i, j, q, r
Molecule details ›
Chains: E, F, N, O, S, U, g, h, i, j, q, r
Length: 419 amino acids
Theoretical weight: 45.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABC7 (Residues: 1-419; Coverage: 100%)
Gene names: JW4132, b4174, hflA, hflK
Sequence domains:
Modulator of FtsH protease HflC Chains: G, H, I, P, Q, R, k, l, m, n, o, p

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.27Å
Relevant EMDB volumes: EMD-32002
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent Advances in Understanding the Structural and Functional Evolution of FtsH Proteases.
Yi et al. (2022)
9 other articles

2 mentions without citation

Are There Lipid Membrane-Domain Subtypes in Neurons with Different Roles in Calcium Signaling?
Samhan-Arias et al. (2023)
1 more