› 7vjh

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-569812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyribodipyrimidine photo-lyase Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.12 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8PYK9 (Residues: 3-464; Coverage: 100%)
Gene name: MM_0852
Sequence domains: DNA photolyase

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand SO4 3 x SO4
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SACLA BEAMLINE BL2
Spacegroup: P43212
Unit cell:
a: 70.52Å b: 70.52Å c: 246.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

5 citation in other articles

Time-resolved serial femtosecond crystallography on fatty-acid photodecarboxylase: lessons learned.
Hadjidemetriou et al. (2022)
4 more