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Structural basis of ALMT1-mediated aluminum resistance in Arabidopsis.

Wang J, Yu X, Ding ZJ, Zhang X, Luo Y, Xu X, Xie Y, Li X, Yuan T, Zheng SJ, Yang W, Guo J
Cell Res 32 89-98 (2022)
Related entries: 7vq3, 7vq4, 7vq5, 7vq7

Cited: 18 times
EuropePMC logo PMID: 34799726

Abstract

The plant aluminum (Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance. Here we present cryo-electron microscopy (cryo-EM) structures of Arabidopsis thaliana ALMT1 (AtALMT1) in the apo, malate-bound, and Al-bound states at neutral and/or acidic pH at up to 3.0 Å resolution. The AtALMT1 dimer assembles an anion channel and each subunit contains six transmembrane helices (TMs) and six cytosolic α-helices. Two pairs of Arg residues are located in the center of the channel pore and contribute to malate recognition. Al binds at the extracellular side of AtALMT1 and induces conformational changes of the TM1-2 loop and the TM5-6 loop, resulting in the opening of the extracellular gate. These structures, along with electrophysiological measurements, molecular dynamic simulations, and mutagenesis study in Arabidopsis, elucidate the structural basis for Al-activated malate transport by ALMT1.

Articles - 7voj mentioned but not cited (2)

  1. Identification of aluminum-activated malate transporters (ALMT) family genes in hydrangea and functional characterization of HmALMT5/9/11 under aluminum stress. Qin Z, Chen S, Feng J, Chen H, Qi X, Wang H, Deng Y. PeerJ 10 e13620 (2022)
  2. Residue-level error detection in cryoelectron microscopy models. Reggiano G, Lugmayr W, Farrell D, Marlovits TC, DiMaio F. Structure 31 860-869.e4 (2023)


Reviews citing this publication (5)

  1. Aluminum in plant: Benefits, toxicity and tolerance mechanisms. Ofoe R, Thomas RH, Asiedu SK, Wang-Pruski G, Fofana B, Abbey L. Front Plant Sci 13 1085998 (2022)
  2. Research Advances in the Mutual Mechanisms Regulating Response of Plant Roots to Phosphate Deficiency and Aluminum Toxicity. Chen W, Tang L, Wang J, Zhu H, Jin J, Yang J, Fan W. Int J Mol Sci 23 1137 (2022)
  3. Aluminum stress signaling, response, and adaptive mechanisms in plants. Liu H, Zhu R, Shu K, Lv W, Wang S, Wang C. Plant Signal Behav 17 2057060 (2022)
  4. Recent Updates on ALMT Transporters' Physiology, Regulation, and Molecular Evolution in Plants. Dabravolski SA, Isayenkov SV. Plants (Basel) 12 3167 (2023)
  5. Why did glutamate, GABA, and melatonin become intercellular signalling molecules in plants? Caspi Y, Pantazopoulou CK, Prompers JJ, Pieterse CMJ, Hulshoff Pol H, Kajala K. Elife 12 e83361 (2023)

Articles citing this publication (11)

  1. DALI shines a light on remote homologs: One hundred discoveries. Holm L, Laiho A, Törönen P, Salgado M. Protein Sci 32 e4519 (2023)
  2. Ammonium Uptake, Mediated by Ammonium Transporters, Mitigates Manganese Toxicity in Duckweed, Spirodela polyrhiza. Kishchenko O, Stepanenko A, Straub T, Zhou Y, Neuhäuser B, Borisjuk N. Plants (Basel) 12 208 (2023)
  3. The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor. Ding ZJ, Xu C, Yan JY, Wang YX, Cui MQ, Yuan JJ, Wang YN, Li GX, Wu JX, Wu YR, Xu JM, Li CX, Shi YZ, Mao CZ, Guo JT, Zhou JM, Benhamed M, Harberd NP, Zheng SJ. Cell Res 34 281-294 (2024)
  4. Uncoupling Aluminum Toxicity From Aluminum Signals in the STOP1 Pathway. Le Poder L, Mercier C, Février L, Duong N, David P, Pluchon S, Nussaume L, Desnos T. Front Plant Sci 13 785791 (2022)
  5. γ-Aminobutyric acid may directly or indirectly regulate Arabidopsis ALMT9. Gilliham M, Xu B. Plant Physiol 190 1570-1573 (2022)
  6. Alluminating structure key to stress tolerance. Gilliham M, Hrmova M. Cell Res 32 5-6 (2022)
  7. Multiple ALMT subunits combine to form functional anion channels: A case study for rice ALMT7. Zhou H, Hu Z, Luo Y, Feng C, Long Y. Front Plant Sci 13 1012578 (2022)
  8. Vacuolar proteomic analysis reveals tonoplast transporters for accumulation of citric acid and sugar in citrus fruit. Mao Z, Wang Y, Li M, Zhang S, Zhao Z, Xu Q, Liu JH, Li C. Hortic Res 11 uhad249 (2024)
  9. Identification of the ALMT gene family in the potato (Solanum tuberosum L.) and analysis of the function of StALMT6/10 in response to aluminum toxicity. Zhang F, Jiang S, Li Q, Song Z, Yang Y, Yu S, Nie Z, Chu M, An Y. Front Plant Sci 14 1274260 (2023)
  10. Picrotoxin Delineates Different Transport Configurations for Malate and γ Aminobutyric Acid through TaALMT1. Ramesh SA, Long Y, Dashtbani-Roozbehani A, Gilliham M, Brown MH, Tyerman SD. Biology (Basel) 11 1162 (2022)
  11. Sensing the toxic aluminum cations in acidic soils. Ryan PR, Yang J. Cell Res 34 269-270 (2024)


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