› 7vud

X-ray diffraction
2.8Å resolution

Carotenoid Cleavage Dioxygenase 1 from Osmanthus fragrans

Released:
DOI: 10.2210/pdb7vud/pdb
PDB entry 7vud coloured by chain, front view.
PDB entry 7vud coloured by chain, side view.
PDB entry 7vud coloured by chain, top view.
Source organism: Osmanthus fragrans
Entry authors: Sharma D, Xue B

Function and Biology Details

Reaction catalysed: 
Zeaxanthin + 2 O(2) = 4,9-dimethyl-dodeca-2,4,6,8,10-pentaen-dial + 2 (3R)-3-hydroxy-beta-ionone
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
carotenoid 9,10-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 63.36 KDa
Source organism: Osmanthus fragrans
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: D4QE74 (Residues: 1-543; Coverage: 100%)
Gene name: CCD1
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

3 bound ligands:
Ligand OXN 3 x OXN
Ligand NI 1 x NI
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P4132
Unit cell:
a: 209.941Å b: 209.941Å c: 209.941Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.258
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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